POU6F1 polyclonal antibody

Product Specifications

Background

Transcription factor that binds preferentially to a variant of the octamer motif (5'-ATGATAAT-3'.

CAS Number

9007-83-4

Structure Composition

1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

POU6F1; BRN5; MPOU; TCFB1

Swiss Prot

Q14863

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein of human POU6F1 (NP_002693.3) .

Conjugation

Unconjugated

Applications

WB, IF/ICC

Dilution

WB,1:500 - 1:2000|IF/ICC,1:50 - 1:100

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

45kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Polyclonal Antibodies

Applications Notes

Western blot analysis of extracts of various cell lines, using POU6F1 antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 90s.