Acetyl-Histone H4-K8 polyclonal antibody

Product Specifications

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.

CAS Number

9007-83-4

Structure Composition

1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

FO108; H4; H4/n; H4F2; H4FN; HIST2H4; Histone H4; HIST1H4A; HIST2H4A

Swiss Prot

P62805

Reactivity

Human, Mouse, Rat, Other (Wide Range)

Immunogen

A synthetic acetylated peptide around K8 of human Histone H4 (NP_003529.1) .

Conjugation

Unconjugated

Applications

WB, IHC, IF/ICC, IP, ChIP, ChIP-seq

Dilution

WB,1:1000 - 1:3000|IHC,1:200 - 1:500|IF/ICC,1:500 - 1:1000|IP,1:200 - 1:500|ChIP,1:20 - 1:50|ChIP-seq,1:20 - 1:50

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .