Acetyl-Histone H3-K27 polyclonal antibody

Product Specifications

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

CAS Number

9007-83-4

Structure Composition

1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

H3.4; H3/g; H3FT; H3t; HIST3H3; Histone H3; HIST1H3A

Swiss Prot

P68431

Reactivity

Human, Mouse, Rat, Other (Wide Range)

Immunogen

A synthetic acetylated peptide around K27 of human Histone H3 (NP_003520.1) .

Conjugation

Unconjugated

Applications

WB, IHC, IF/ICC, IP, ChIP, ChIP-seq

Dilution

WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200|IP,1:50 - 1:200|ChIP,1:20 - 1:100|ChIP-seq,1:20 - 1:100

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .