IDH2 polyclonal antibody

Product Specifications

Background

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD (+) as the electron acceptor and the other NADP (+) . Five isocitrate dehydrogenases have been reported: three NAD (+) -dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP (+) -dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP (+) -dependent isozyme is a homodimer. The protein encoded by this gene is the NADP (+) -dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants.

CAS Number

9007-83-4

Structure Composition

1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

IDH2; D2HGA2; ICD-M; IDH; IDHM; IDP; IDPM; mNADP-IDH

Swiss Prot

P48735

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein of human IDH2 (NP_002159.2) .

Conjugation

Unconjugated

Applications

WB, IHC, IF/ICC, IP, ChIP

Dilution

WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:20 - 1:50|IP,1:50 - 1:200|ChIP,1:20 - 1:100

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .