SAP18 polyclonal antibody
Product Specifications
Background
Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Histone acetylation and deacetylation are catalyzed by multisubunit complexes. The protein encoded by this gene is a component of the histone deacetylase complex, which includes SIN3, SAP30, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. This protein directly interacts with SIN3 and enhances SIN3-mediated transcriptional repression when tethered to the promoter. A pseudogene has been identified on chromosome 2.
CAS Number
9007-83-4
Structure Composition
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
SAP18;2HOR0202; SAP18P
Swiss Prot
O00422
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein of human SAP18 (NP_005861.2) .
Conjugation
Unconjugated
Applications
WB, IHC, IF/ICC
Dilution
WB,1:200 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
18kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Polyclonal Antibodies
Applications Notes
Western blot analysis of extracts of SW480 cells, using SAP18 antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 90s.
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
MBS1381593-01 0.02 mg (E-Coli)
Recombinant Streptococcus pyogenes serotype M1 Methylenetetrahydrofolate--tRNA- (uracil-5-) -methyltransferase TrmFO (trmFO)
Sign In for Pricing
View Details MBS1381593-02 0.02 mg (Yeast)
Recombinant Streptococcus pyogenes serotype M1 Methylenetetrahydrofolate--tRNA- (uracil-5-) -methyltransferase TrmFO (trmFO)
Sign In for Pricing
View Details MBS1381593-03 0.1 mg (E-Coli)
Recombinant Streptococcus pyogenes serotype M1 Methylenetetrahydrofolate--tRNA- (uracil-5-) -methyltransferase TrmFO (trmFO)
Sign In for Pricing
View Details MBS1381593-04 0.1 mg (Yeast)
Recombinant Streptococcus pyogenes serotype M1 Methylenetetrahydrofolate--tRNA- (uracil-5-) -methyltransferase TrmFO (trmFO)
Sign In for Pricing
View Details MBS1381593-05 0.02 mg (Baculovirus)
Recombinant Streptococcus pyogenes serotype M1 Methylenetetrahydrofolate--tRNA- (uracil-5-) -methyltransferase TrmFO (trmFO)
Sign In for Pricing
View Details MBS1381593-06 0.02 mg (Mammalian-Cell)
Recombinant Streptococcus pyogenes serotype M1 Methylenetetrahydrofolate--tRNA- (uracil-5-) -methyltransferase TrmFO (trmFO)
Sign In for Pricing
View Details