U2AF2/U2AF65 polyclonal antibody

Product Specifications

Background

U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date.

CAS Number

9007-83-4

Structure Composition

1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

U2AF2; U2AF65

Swiss Prot

P26368

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein of human U2AF2/U2AF65 (NP_001012496.1) .

Conjugation

Unconjugated

Applications

WB, IF/ICC, IP

Dilution

WB,1:500 - 1:2000|IF/ICC,1:50 - 1:100|IP,1:50 - 1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .