Bmi1 polyclonal antibody

Product Specifications

Background

This gene encodes a ring finger protein that is major component of the polycomb group complex 1 (PRC1) . This complex functions through chromatin remodeling as an essential epigenetic repressor of multiple regulatory genes involved in embryonic development and self-renewal in somatic stem cells. This protein also plays a central role in DNA damage repair. This gene is an oncogene and aberrant expression is associated with numerous cancers and is associated with resistance to certain chemotherapies. A pseudogene of this gene is found on chromosome X. Read-through transcription also exists between this gene and the upstream COMM domain containing 3 (COMMD3) gene.

CAS Number

9007-83-4

Structure Composition

1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

BMI1; FLVI2/BMI1; PCGF4; RNF51; flvi-2/bmi-1; Bmi1

Swiss Prot

P35226

Reactivity

Human

Immunogen

Recombinant fusion protein of human Bmi1 (NP_005171.4) .

Conjugation

Unconjugated

Applications

WB, IF/ICC

Dilution

WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

41kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Polyclonal Antibodies

Applications Notes

Western blot analysis of extracts of U-937 cells, using Bmi1 antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 30s.