Grp94 polyclonal antibody

Product Specifications

Background

This gene encodes a member of a family of adenosine triphosphate (ATP) -metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15.

CAS Number

9007-83-4

Structure Composition

1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

HSP90B1; ECGP; GP96; GRP94; HEL-S-125m; HEL35; TRA1

Swiss Prot

P14625

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide of human Grp94 (NP_003290.1) .

Conjugation

Unconjugated

Applications

WB, IHC, IF/ICC

Dilution

WB,1:500 - 1:2000|IHC,1:50 - 1:100|IF/ICC,1:50 - 1:100

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

110kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Polyclonal Antibodies

Applications Notes

Immunohistochemistry of paraffin-embedded rat testis using Grp94 antibody at dilution of 1:200 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.