CD161 (3F8) monoclonal antibody

Product Specifications

Background

Natural killer (NK) and T cells express a superfamily of proteins with structural features of C-type lectins. T cells bearing natural killer receptors (NKRs) such as CD94 and CD161 are present in psoriasis. CD161 mediates NK cell activation and functions as an activating receptor. CD161 is a prototypic marker of NK cells, although it is also found on a subset of CD8+ T cells. The expression of NK receptors on CD8+ T cells can be considered a marker of cytotoxic effector T cells that are expanded in vivo after antigenic activation leading to extensive proliferation. The transcription, mRNA accumulation, and surface expression of CD161, a molecule involved in triggering cytotoxicity, is specifically upregulated by IL-12.

CAS Number

9007-83-4

Structure Composition

1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Killer cell lectin-like receptor subfamily B member 1; C-type lectin domain family 5 member B; HNKR-P1a; NKR-P1A; Natural killer cell surface protein P1A; CD161

Swiss Prot

Q12918

Reactivity

Rat

Immunogen

Synthetic peptide, corresponding to amino acids of Human CD161.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:2000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 25 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

CD161 (3F8) mAb detects endogenous levels of CD161 protein.

Applications Notes

Western blot (WB) analysis of CD 161 (NKR-PIA) mAb at 1:2000 dillution Lane1:The Peripheral blood lysate of Rat (40μg) Lane2:The Liver tissue lysate of Rat (40μg)