V5-tag monoclonal antibody
Product Specifications
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.
CAS Number
9007-83-4
Structure Composition
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
V5tag
Reactivity
Transfected
Immunogen
Synthetic peptide, corresponding to V5 tag.
Conjugation
Unconjugated
Applications
WB IP
Dilution
WB: 1:5000~1:20000 IP: 1:100~1:1000
Purity
The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 95 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
V5 mAb detects endogenous levels of V5 protein.
Applications Notes
Western blot (WB) analysis of V5 mAb at 1:5000 dilution Lane1:HEK293T whole cell lysate (40ug) Lane2:HEK293T transfected with pcDNA3.1-MCM5-V5-His (48h) whole cell lysate (10ug) Lane3:HEK293T transfected with pcDNA3.1-MCM5-V5-His (48h) whole cell lysate (20ug) Lane4:HEK293T transfected with pcDNA3.1-MCM5-V5-His (48h) whole cell lysate (30ug) Lane5:HEK293T transfected with pcDNA3.1-MCM5-V5-His (48h) whole cell lysate (40ug)
Host or Source
Mouse
Available Sizes
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