EGFRvIII (2C10) monoclonal antibody

Product Specifications

Background

EGFR variant III (EGFRvIII) is a truncated, constitutively active mutant form of EGFR that results from an in-frame deletion of exons 2-7. EGFRvIII is expressed in various cancers, most notably glioblastoma, where it is expressed at a frequency of 25-30%. Although complicated by the fact that it is often co-expressed with amplified EGFR, EGFRvIII is a potential therapeutic target.

CAS Number

9007-83-4

Structure Composition

1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

EGFR vIII; EGFR class lII variant; EGF Receptor vIII; EGFR variant III

Swiss Prot

P00533

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to residues in Human EGFRvIII Peptide (LEEKKGNYVVTDHC) .

Conjugation

Unconjugated

Related Pathways

ErbB HER Signaling

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 170 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

EGFR vlII mAb detects endogenous levels of EGFR vlII protein.

Applications Notes

Western blot (WB) analysis of EGFR vlII mAb at 1:1000 dilution Lane1:A549 whole cell lysate (40ug) Lane2:SGC7901 whole cell lysate (40ug) Lane3:NIH-3T3 whole cell lysate (40ug) Lane4:C6 whole cell lysate (40ug)