VSV-G-Tag Mouse monoclonal antibody

Product Specifications

Background

Epitope tagging is a widely accepted technique that involves the fusion of an epitope labeled peptide to a certain protein as a marker for gene expression. This technique allows gene expression to be easily monitored during western blotting, immunoprecipitation and immunofluorescence by using an antibody that recognizes such an epitope. Amino acid sequences that are widely used for epitope tagging are as follows; YPYDVPDYA (HA-Tag), EQKLISEEDL (Myc-Tag) and YTDIEMNRLGK (VSV-G-Tag), which correspond to the partial peptide of Influenza hemagglutinin protein, human c-myc gene product, and Vesicular stomatitis virus glycoprotein, respectively.

CAS Number

9007-83-4

Structure Composition

Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

VSVG

Reactivity

Transfected

Immunogen

Synthetic peptide VSV-G-Tag (YTDIEMNRLGK) .

Conjugation

Unconjugated

Applications

WB IF

Dilution

WB: 1:5000~1:10000 IF: 1:200

Purity

Affinity-chromatography

Modification

Unmodification

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

VSV-G-tag mouse monoclonal antibody detects endogenous levels of internal, C-terminal, and N-terminal VSV-G- tagged proteins.

Applications Notes

IP antibody use:5ug VSV-G Mouse IgG1 per ml Lysate, WB 1:5000 1.untransfected 293 cell lysate 2.transfected 293 cell lysate with VSV-G-tag fusion protein 3.IP (untransfted 293+anti-VSV-G mAb+Protein G agarose) 4.IP (transfected 293+ normal Mouse IgG+Pr