T7-Tag Mouse monoclonal antibody
Product Specifications
Background
The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors that is based on the very efficient T7 RNA polymerase expression system. Antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.
CAS Number
9007-83-4
Structure Composition
Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
T7 Tag; MASMTGGQQMG epitope tag; Enterobacteria phage T7; Bacteriophage T7; Minor capsid protein 10B; Major capsid protein 10A
Reactivity
Transfected
Immunogen
Synthetic peptide T7-Tag.
Conjugation
Unconjugated
Applications
WB IF
Dilution
WB: 1:5000~1:10000 IF: 1:200
Purity
Affinity-chromatography
Modification
Unmodification
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
T7-tag mouse monoclonal antibody detects endogenous levels of internal, C-terminal, and N-terminal T7- tagged proteins.
Applications Notes
Western blot analysis of GST-T7 fusion protein, using T7-tag mouse monoclonal antibody.
Host or Source
Mouse
Available Sizes
Frequently Asked Questions
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