Histone H2A.X (phospho-S139) monoclonal antibody

Product Specifications

Background

H2A.X is a member of the histone H2A family, 1 of 5 families of histone proteins involved in nucleosomal organization of chromatin. H2A.X is synthesised in G1 as well as S phase and is known to be important for recombination between immunoglobulin switch regions. A very early step in the response of mammalian cells to DNA double-strand breaks is the phosphorylation of histone H2A.X at serine 139 at the sites of DNA damage. There is a predicted acetylation at residue 1 and ubiquitination at reside 119. Phosphorylated H2A.X promotes DNA repair and maintains genomic stability.

CAS Number

9007-83-4

Structure Composition

1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Histone H2AX; H2a/x; Histone H2A.X; H2AFX; H2AX; γ-H2AX; γH2AX; ganunaH2AX; γ-H2A.X; γH2A.X; ganunaH2A.X

Swiss Prot

P16104

Reactivity

Human, Mouse

Immunogen

Synthetic phosphopeptide derived from human Histone H2A.X around the phosphorylation site of Serine 139.

Conjugation

Unconjugated

Related Pathways

Regulation of Apoptosis, Histone Lysine Methylation Pathway

Applications

WB IF

Dilution

WB: 1:1000~2000 IF: 1:200~500

Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

Predicted band size:15KDa Observed band size:15KDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

This antibody detects endogenous levels of Histone H2A.X protein only when phosphorylated at Ser139, and does not cross-react with related proteins.

Applications Notes

Western blot detection of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cell lysates using Phospho-Histone H2A.X (Ser139) mouse mAb (1:2000 diluted) .