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MPI monoclonal antibody

Product Specifications

Background

Phosphomannose isomerase catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate and plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib.Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.

CAS Number

9007-83-4

Structure Composition

Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Phosphohexomutase; Phosphomannose isomerase; PMI; PMI1; CDG1B

Swiss Prot

P34949

Reactivity

Human, Rat

Immunogen

Recombinant full length Human MPI.

Conjugation

Unconjugated

Applications

WB ICC

Dilution

WB: 1:500~2000 ICC: 1:200~500

Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

Predicted band size:54KDa Observed band size:45KDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

This antibody detects endogenous levels of Mannose Phosphate Isomerase and does not cross-react with related proteins

Applications Notes

Western blot detection of MPI in Rat kidney, Rat brain, A549 & LNCAP cell lysates and using MPI antibody (1:1000 diluted) .

Host or Source

Mouse

Available Sizes

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