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USP7 (C-terminus) monoclonal antibody

Product Specifications

Background

Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme (DUB) action. Five DUB subfamilies are recognized, including the USP, UCH, OTU, MJD and JAMM enzymes. Herpesvirus-associated ubiquitin-specific protease (HAUSP, USP7) is an important deubiquitinase belonging to USP subfamily. A key HAUSP function is to bind and deubiquitinate the p53 transcription factor and an associated regulator protein Mdm2, thereby stabilizing both proteins. In addition to regulating essential components of the p53 pathway, HAUSP also modifies other ubiquitinylated proteins such as members of the FoxO family of forkhead transcription factors and the mitotic stress checkpoint protein CHFR.

CAS Number

9007-83-4

Structure Composition

1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

TEF1; HAUSP; Ubiquitin carboxyl-terminal hydrolase 7; Deubiquitinating enzyme 7; Ubiquitin thioesterase 7; Ubiquitin-specific-processing protease 7

Swiss Prot

Q93009

Reactivity

Human

Immunogen

Synthetic peptide, corresponding to the C-terminus of Human USP7o.

Conjugation

Unconjugated

Applications

WB ICC

Dilution

WB: 1:1000 ICC: 1:100~200

Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

Predicted band size:129KDa Observed band size:140KDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

This antibody detects endogenous levels of USP7 (C-terminus) and does not cross-react with related proteins

Applications Notes

Western blot detection of USP7 (C-terminus) antibody in Hela cell lysates using USP7 (C-terminus) antibody (1:1000 diluted) .

Host or Source

Mouse

Available Sizes

Frequently Asked Questions

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