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Smad5 (C-terminus) monoclonal antibody

Product Specifications

Background

Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MADH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members.

CAS Number

9007-83-4

Structure Composition

1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Mothers against decapentaplegic homolog 5; MAD homolog 5; Mothers against DPP homolog 5; JV5-1; SMAD family member 5; SMAD 5; Smad5; hSmad5; SMAD5; MADH5

Swiss Prot

Q99717

Reactivity

Human, Mouse

Immunogen

Synthetic peptide, corresponding to the C-terminus of Human Smad5.

Conjugation

Unconjugated

Related Pathways

Contribution of Soluble Factors to EMT, hippo Signaling, TGFβ Smad signaling

Applications

WB ICC FC

Dilution

WB: 1:1000 ICC: 1:50~100 FC: 1:50~100

Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

Predicted band size:55KDa Observed band size:55KDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

This antibody detects endogenous levels of SMAD5 (C-terminus) and does not cross-react with related proteins

Applications Notes

Western blot detection of SMAD5 antibody in Mouse testis cell lysates using SMAD5 antibody (1:1000 diluted) .

Host or Source

Mouse

Available Sizes

Frequently Asked Questions

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