Cdk1/Cdc2 monoclonal antibody
Product Specifications
Background
Cdc2, an evolutionarily conserved serine/threonine-specific protein kinase, is essential in the cell cycle transition from G2 to M phase. Cdc2 is regulated by association with B-type cyclins and by reversible phosophorylation. Cyclin B binding facilitates the phosphorylation of Cdc2 p34 on three regulatory sites: threonine 14, tyrosine 15, and threonine 161. In higher eukaryotes, Cdc2 is negatively regulated by phosphorylation of two residues located in the ATP-binding site, Thr 14 and Tyr 15. Cdc2 is positively regulated by the cyclin-dependent phosphorylation of Thr 161. Both phosphorylation and de- phosphorylation at Thr 161 are required for progression through the cell cycle.
CAS Number
9007-83-4
Structure Composition
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Cyclin-dependent kinase 1; Cell division control protein 2 homolog; Cell division protein kinase 1; p34 protein kinase; CDK1; CDC2; CDC28A; CDKN1; P34CDC2
Swiss Prot
P06493
Reactivity
Human
Immunogen
Recombinant full length Human Cdk1/Cdc2.
Conjugation
Unconjugated
Related Pathways
Cell Cycle G2 M DNA Damage Signaling Pathway
Applications
WB ICC
Dilution
WB: 1:1000 ICC: 1:50~200
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
Predicted band size:34KDa Observed band size:34KDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
This antibody detects endogenous levels of Cdk1/Cdc2 and does not cross-react with related proteins
Applications Notes
Western blot detection of CDC2/CDK1 in MCF7&Hela cell lysates using CDC2/CDK1 antibody (1:1000 diluted) .
Host or Source
Mouse
Available Sizes
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