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Tubulin β monoclonal antibody

Product Specifications

Background

Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells.

CAS Number

9007-83-4

Structure Composition

1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Total tubulin β; Tubulin beta

Swiss Prot

Q13509/P04350/P68371/P07437/Q13885/Q9BUF5

Reactivity

Human, Mouse, Rat, Monkey, Goat, Hamster

Immunogen

Recombinant full length Human Tubulin beta.

Conjugation

Unconjugated

Related Pathways

Regulation of Microtubule Dynamics

Applications

WB ICC

Dilution

WB: 1:1000 ICC: 1:200~1000

Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

Predicted band size:55KDa Observed band size:55KDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

This antibody detects endogenous levels of Tubulin beta and does not cross-react with related proteins.

Applications Notes

Western blot detection of TUBB in COS7, LNCap, MCF7&C2C12 cell lysates and using TUBB antibody (1:5000 diluted) . Predicted band size: 55KDa.

Host or Source

Mouse

Available Sizes

Frequently Asked Questions

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