Lad (I249) polyclonal antibody

Product Specifications

Background

Lad (TSAD, TSAd, F2771, SH2D2A, SH2 domain protein 2A) is a 52 kDa cytoplasmic adapter protein that undergoes tyrosine-phosphorylation and influences T cell activation. Lad (SH2D2A) mRNA is present in peripheral blood leukocytes, thymus and spleen, and accumulates in the cytoplasm during T cell activation. The Lad gene maps to chromosome 1q21 in a region where alterations are characteristic to lymphomas.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Ladinin-1; Lad-1; Linear IgA disease antigen (LADA) ; LAD1; LAD

Swiss Prot

O00515

Reactivity

Human

Immunogen

Synthetic peptide, corresponding to amino acids 218-260 of Human Lad.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 54 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Lad (I249) polyclonal antibody detects endogenous levels of Lad protein.

Applications Notes

Western blot (WB) analysis of Lad (I249) polyclonal antibody at 1:500 dilution Lane1:K562 whole cell lysate (40ug) Lane2:HUT78 whole cell lysate (40ug)