Osteopontin polyclonal antibody

Product Specifications

Background

Osteopontin (OPN), also designated bone sialoprotein 1, urinary stone protein, spp-1, Eta-1, nephropontin and uropontin, is an extracellular matrix cell adhesion phosphoglycoprotein. OPN is deposited into unmineralized matrix prior to calcification leading to localization at various tissue interfaces including cement lines, lamina limitans and between collagen fibrils of fully matured hard tissues. While OPN is a major product of osteoblasts, it is also synthesized by brain and kidney cells. OPN isolated from or secreted by various tissues ranges in molecular weight due to posttranslational modifications. OPN functions as a substrate for transglutaminase and is involved in cell adhesion, chemoattraction and immunomodulation.

CAS Number

9007-83-4

Structure Composition

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Product Name Alternative

SPP-1, SPP1, BNSP, OPN, PSEC0156, BNSP, Bone sialoprotein 1, BSP I, BSPI, Early T lymphocyte activation 1, ETA 1, ETA1, MGC110940, Nephropontin, OPN, Osteopontin, osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein, OSTP, PSEC0156, secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1), Secreted phosphoprotein 1, SPP 1, SPP-1, SPP1, SPP1/CALPHA1 fusion, Urinary stone protein, Uropontin

Swiss Prot

P10451

Reactivity

Human, Mouse, Rat

Immunogen

KLH conjugated synthetic peptide derived from mouse OPN: 221-280/294

Conjugation

Unconjugated

Applications

WB IHC IF ELISA

Dilution

WB:1:500-1:2000 IHC:1:100-1:500 IF:1:100-1:500 ELISA:1:5,000-1:10,000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .