NEDD4L (phospho-S448) polyclonal antibody
Product Specifications
Background
This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
HNEDD4-2; NEDD4-2; NEDD4.2; PVNH7; RSP5; hNEDD4-2; NEDD4-2; NEDD4.2; RSP5
Swiss Prot
Q96PU5
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic phosphorylated peptide around S448 of human NEDD4L
Conjugation
Unconjugated
Applications
WB
Dilution
WB 1:500 - 1:2000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
130kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
NEDD4L (phospho-S448) polyclonal antibody detects endogenous levels of NEDD4L protein when phosphorylated at Ser448.
Applications Notes
Western blot analysis of extracts of various cell lines, using Phospho-NEDD4L-S448 antibody.
Host or Source
Rabbit
Available Sizes
Curated Selection
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