CAF-1 p60 polyclonal antibody
Product Specifications
Background
Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication. The p150 subunit of CAF-1 also supports the maintenance of heterochromatin, which requires the synthesis of both new histones and heterochromatin proteins and their orderly assembly during DNA replication.
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Chromatin assembly factor 1 subunit B; CAF-1 subunit B; Chromatin assembly factor I p60 subunit; CAF-I 60 kDa subunit; CAF-I p60; M-phase phosphoprotein 7; CHAF1B; CAF1A; CAF1P60; MPHOSPH7; MPP7; CAF1 p60; CAF1p60; p60 CAF1; p60-CAF1
Swiss Prot
Q13112
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant protein of human CHAF1B
Conjugation
Unconjugated
Applications
WB IHC IF
Dilution
WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:20 - 1:100
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
70kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
CAF-1 p60 polyclonal antibody detects endogenous levels of CAF-1 p60 protein.
Applications Notes
Western blot analysis of extracts of various cell lines, using CHAF1B antibody at 1:1000 dilution.
Host or Source
Rabbit
CAS Number
9007-83-4
Available Sizes
Curated Selection
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