Actived-Caspase-9 p35 (D315) polyclonal antibody
Product Specifications
Background
Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family. Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf 1. Apaf-1 mediated activation of caspase-9 involves instrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
APAF 3, APAF-3, APAF3, Apoptosis related cysteine peptidase, Apoptotic protease activating factor 3, Apoptotic protease Mch 6, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, CASP 9, CASP-9, CASP9, Caspase 9 apoptosis related cysteine peptidase, Caspase 9 Dominant Negative, Caspase 9c, Caspase-9, Caspase9, ICE LAP6, ICE like apoptotic protease 6, ICE-LAP6, ICE-like apoptotic protease 6, MCH6, RNCASP9
Swiss Prot
P55211
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 300-350 of Human Caspase 9.
Conjugation
Unconjugated
Related Pathways
Regulation of Apoptosis, Inhibition of Apoptosis, Mitochondrial Control of Apoptosis, Death Receptor Signaling
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 35 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Actived-Caspase-9 p35 (D315) polyclonal antibody detects detects endogenous levels of the 35 kDa large fragment of caspase-9 following cleavage at aspartic acid 315.
Applications Notes
Western blot (WB) analysis of Actived-Caspase-9 p35 (D315) polyclonal antibody at 1:1000 dilution Lane1:Hela whole cell lysate (40ug) Lane2:Hela treated with H2O2 (100nmol/ml, 25min, Na3O4V) whole cell lysate (40ug) Lane3:Hela treated with H2O2 (100nmol/ml, 25min, λ-ppase) whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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