Cleaved-Ephrin-A2 (N188) polyclonal antibody

Product Specifications

Background

The Eph subfamily represents the largest group of receptor protein kinases identified to date. There is increasing evidence that Eph family members are involved in central nervous system function and in development. Ligands for Eph receptors include ephrin-A1 (LERK-1/B61), identified as a ligand for the EphA2 (Eck) receptor; ephrin-A2 (ELF-1), identified as a ligand for the EphA3 and EphA4 (Sek) receptors; ephrin-A3 (LERK-3), identified as a ligand for EphA5 (Ehk1) and EphA3 (Hek) receptors; ephrin-A4 (LERK-4), identified as a ligand for the EphA3 receptor; ephrin-A5 (AL-1), identified as a ligand for EphA5 (REK7) ; ephrin-B1 (LERK-2), identified as a ligand for the EphB1 (Elk) and EphB2 (Cek5) receptors; ephrin-B2 (LERK-5), identified as a ligand for the EphB1, EphB3 (Cek10) and EphB2 receptors; and ephrin-B3 (LERK-8), identified as a ligand for EphB1.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Ephrin A2; EPH-related receptor tyrosine kinase ligand 6; LERK-6; HEK7 ligand; HEK7-L; EFNA2; EPLG6; LERK6

Swiss Prot

O43921

Reactivity

Human

Immunogen

Synthetic peptide, corresponding to amino acids 161-210 of Human Ephrin-A2.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Cleaved

Molecular Weight

~ 21,24 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Cleaved-Ephrin-A2 (N188) polyclonal antibody detects endogenous levels of Cleaved-Ephrin-A2 (21 kDa) protein.

Applications Notes

Western blot (WB) analysis of Cleaved-Ephrin-A2 (N188) pAb at 1:500 dilution Lane1:U-87MG whole cell lysate (40ug) Lane2:HCT116 whole cell lysate (40ug) Lane3:SGC7901 whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug) Lane5:HepG2 whole cell lysate (40ug)