Cleaved-Notch 1 (V1754) polyclonal antibody

Product Specifications

Background

Notch1 is a transmembrane protein functioning in development and the determination of cell fate. During maturation, the notch molecule is cleaved by a furin-like convertase at its extracellular domain. Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the carboxy-terminal notch1 fragment is released and further cleaved between Gly1753 and Val1754. The resulting activated cytosolic fragment translocates to the nucleus where it activates transcription.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Neurogenic locus notch homolog protein 1; Notch 1; hN1; Translocation-associated notch protein TAN-1; Notch 1 intracellular domain; NICD; NOTCH1; TAN1

Swiss Prot

P46531

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 1700-1800 of Human Notch 1.

Conjugation

Unconjugated

Related Pathways

Neuronal and Glial Cell Marker Atlas, Notch Signaling, Tumor Angiogenesis, Contribution of Soluble Factors to EMT

Applications

IHC

Dilution

IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Cleaved

Molecular Weight

~ 110 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Cleaved-Notch 1 (V1754) polyclonal antibody detects endogenous levels of the Notch1 intracellular domain (NICD) . The antibody does not recognize full-length Notch1.