Caspase-6 p18 (D162) polyclonal antibody

Product Specifications

Background

Caspase-6 (Mch2) is one of the major executioner caspases functioning in cellular apoptotic processes. Upon apoptotic stimulation, initiator caspases such as caspase-9 are cleaved and activated. The activated upstream caspases further process downstream executioner caspases, such as caspase-3 and caspase-6, by cleaving them into large and small subunits, thereby initiating a caspase cascade leading to apoptosis. One of the major targets for caspase-6 is the membrane associated protein lamin A. The cleavage of this protein causes cell membrane malfunction, membrane blebbing and eventual cell death.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

MCH2; CASP6; Caspase-6; Caspase 6; Caspase6

Swiss Prot

O08738

Reactivity

Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 141-190 of Human Caspase-6.

Conjugation

Unconjugated

Related Pathways

Inhibition of Apoptosis

Applications

IHC

Dilution

IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 18, 35 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Caspase-6 p18 (D162) polyclonal antibody detects endogenous levels of the large subunit of active caspase-6 resulting from cleavage at Asp162 in mouse and rat (18 kDa) . This antibody does not recognize other cleaved caspases.