Histone H3 (Acetyl-K23) polyclonal antibody

Product Specifications

Background

Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27 and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10, Ser28 and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. Phosphorylation of Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation of H3 Thr3 in prophase and its dephosphorylation during anaphase.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Histone H3.1; HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.2; Histone H3/m; Histone H3/o; HIST2H3A; HIST2H3C; H3F2; H3FM; HIST2H3D; Histone H3.3; H3F3A; H3.3A; H3F3; H3F3B; H3.3B

Swiss Prot

P68431/Q71DI3/P84243

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide including acetyl-lysine 23 of Histone H3.

Conjugation

Unconjugated

Related Pathways

Histone Lysine Methylation Pathway, Examples of Crosstalk Between Post-translational Modifications, DNA Methylation

Applications

WB IF

Dilution

WB: 1:500~1:1000 IF: 1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Acetylated

Molecular Weight

~ 17 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Ac-Histone H3 (K23) polyclonal antibody detects endogenous levels of Histone H3 protein only when acetylated at Lys23.

Applications Notes

Western blot analysis of extracts from Raw264.7 cells, treated with TSA 400nM 24h, using Histone H3 (Acetyl-K23) Antibody. The lane on the left is treated with the synthesized peptide.