CARS polyclonal antibody

Aminoacyl-tRNA synthetases consist of a family of enzymes that catalyze the specific aminoacylation of tRNA by their cognate amino acid in the initial step of ribosome-dependent protein biosynthesis. Cytoplasmic CysRS (Cysteinyl-tRNA synthetase, cytoplasmic), also known as CARS, is a 748 amino acid member of the class-I aminoacyl-tRNA synthetase protein family. Cytoplasmic CysRS is a monomeric protein that binds one zinc ion per subunit for use as a cofactor. Cytoplasmic CysRS uses ATP to convert L-cysteine and tRNA (Cys) into ADP, a diphosphate and L-cysteinyl-tRNA (Cys) . A chromosomal aberration of the gene that encodes Cytoplasmic CysRS is associated with inflammatory myofibroblastic tumors (IMTs) . Cytoplasmic CysRS is expressed as two isoforms produced by alternative splicing events.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .