CARS polyclonal antibody

Product Specifications

Background

Aminoacyl-tRNA synthetases consist of a family of enzymes that catalyze the specific aminoacylation of tRNA by their cognate amino acid in the initial step of ribosome-dependent protein biosynthesis. Cytoplasmic CysRS (Cysteinyl-tRNA synthetase, cytoplasmic), also known as CARS, is a 748 amino acid member of the class-I aminoacyl-tRNA synthetase protein family. Cytoplasmic CysRS is a monomeric protein that binds one zinc ion per subunit for use as a cofactor. Cytoplasmic CysRS uses ATP to convert L-cysteine and tRNA (Cys) into ADP, a diphosphate and L-cysteinyl-tRNA (Cys) . A chromosomal aberration of the gene that encodes Cytoplasmic CysRS is associated with inflammatory myofibroblastic tumors (IMTs) . Cytoplasmic CysRS is expressed as two isoforms produced by alternative splicing events.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Cysteine--tRNA ligase, cytoplasmic; Cysteinyl-tRNA synthetase; CysRS; CARS

Swiss Prot

P49589

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant full length Human CARS.

Conjugation

Unconjugated

Applications

WB IHC IF

Dilution

WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .