PKM2 polyclonal antibody

Product Specifications

Background

The PKM2 (pyruvate kinase, muscle) gene encodes the M1- and M2-type isoenzymes through alternative splicing events. Both M1- and M2-type isoforms exists as tetramers and are stimulated by fructose 1,6-bisphosphate. In addition, both isoforms exhibit thyroid hormone binding activity and may be referred to as CTHBP (cytosolic thyroid hormone-binding protein) or THBP1.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

CTHBP; Cytosolic thyroid hormone binding protein; Cytosolic thyroid hormone-binding protein; KPYM; MGC3932; OIP 3; OIP-3; Oip3; OIP3; OPA interacting protein 3; Opa-interacting protein 3; p58; PK Muscle type; PK; muscle type; PK2; Pk3; PKM; PKM2; PYKM; Pyruvate kinase 2/3; Pyruvate kinase isozymes M1/M2; Pyruvate kinase muscle; Pyruvate kinase muscle isozyme; TCB; THBP1; Thyroid hormone binding protein 1; Thyroid hormone binding protein cytosolic; Thyroid hormone

Swiss Prot

P14618

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant full length Human PKM2.

Conjugation

Unconjugated

Related Pathways

Warburg Effect, Insulin &Glucose Signaling, Glutamine Metabolism

Applications

WB IHC IF IP RIP

Dilution

WB: 1:500~1:2000 IHC/IF: 1:50~1:200 IP/RIP: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 58 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

PKM2 polyclonal antibody detects endogenous levels of PKM2 protein.

Applications Notes

Western blot (WB) analysis of PKM2 polyclonal antibody at 1:500 dilution Lane1:C6 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:The stomach tissue lysate of Mouse (40ug) Lane4:MCF-7 whole cell lysate (20ug)