PTK2 (phospho-S843) polyclonal antibody

Product Specifications

Background

Focal adhesion kinase was initially identified as a major 125 kDa substrate for the intrinsic protein tyrosine kinase activity of Src-encoded pp60. The deduced amino acid sequence of FAK p125 has shown it to be a cytoplasmic protein tyrosine kinase whose sequence and structural organization are unique as compared to other proteins described to date. Localization of p125 by immunofluorescence suggests that it is primarily found in cellular focal adhesions leading to its designation as focal adhesion kinase (FAK) . FAK is concentrated at the basal edge of only basal keratinocytes that are actively migrating and rapidly proliferating in repairing burn wounds, and is activated and localized to the focal adhesions of spreading keratinocytes in culture.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Focal adhesion kinase 1; FADK 1; Focal adhesion kinase-related nonkinase; FRNK; Protein phosphatase 1 regulatory subunit 71; PPP1R71; Protein-tyrosine kinase 2; p125FAK; pp125FAK; PTK2; FAK; FAK1

Swiss Prot

Q05397

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant full length Human PTK2.

Conjugation

Unconjugated

Related Pathways

Regulation of Actin Dynamics, Tumor Angiogenesis, Contribution of ECM and Cytoskeletal Factors to EMT, G Protein-coupled Receptors Signaling to MAPK Erk

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 125 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -22°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

PTK2 (phospho-S843) polyclonal antibody detects endogenous levels of PTK2 protein only when phosphorylated at Ser843.

Applications Notes

Western blot (WB) analysis of PTK2 (phospho-S843) polyclonal antibody at 1:500 dilution Lane1:SK-OVCAR3 whole cell lysate (40µg) Lane2:LOVO whole cell lysate (40µg) Lane3:A549 whole cell lysate (40µg) Lane4:HepG2 whole cell lysate (40µg) Lane5:Hela whole cell lysate (40µg) Lane6:3T3-L1 whole cell lysate (40µg) Lane7:CT-26 whole cell lysate (40µg)