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MBP (phospho-T232) polyclonal antibody

Product Specifications

Background

Myelin basic protein (MBP) is an abundant central nervous system (CNS) myelin membrane protein that plays an important role in nerve myelination. Myelin sheaths are multi-layered membranes derived from oligodendrocytes that increase the conduction velocity of axonal impulses. MBP helps to adhere the cytoplasmic leaflets of adjacent oligodendrocyte membranes to one another. Several splice variants of MBP are expressed in cells and the protein is modified following translation (i.e. phosphorylation, methylation), which suggests additional membrane adhesion functions.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Myelin basic protein; MBP; Myelin A1 protein; Myelin membrane encephalitogenic protein; MBP

Swiss Prot

P02686

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human MBP around the phosphorylation site of Threonine 232.

Conjugation

Unconjugated

Related Pathways

Neuronal and Glial Cell Marker Atlas

Applications

WB

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 12-18 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

MBP (phospho-T232) polyclonal antibody detects endogenous levels of MBP protein only when phosphorylated at T232..

Applications Notes

Western blot (WB) analysis of MBP (phospho-T232) polyclonal antibody at 1:500 dilution Lane1:The Brain tissue lysate of Mouse (20ug) Lane2:The Brain tissue lysate of Rat (20ug)

Host or Source

Rabbit

Available Sizes

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