NOS1 (phospho-S1417) polyclonal antibody

Product Specifications

Background

Nitric Oxide Synthase (NOS) catalyzes the formation of nitric oxide (NO) and citruline from L-arginine, oxygen and cofactors. Three family members have been characterized: neuronal NOS (nNOS), which is found primarily in neuronal tissue; inducible NOS (iNOS), which is induced by interferon gamma and lipopolysaccharides in the kidney and cardiovascular system; and endothelial NOS (eNOS), which is expressed in blood vessels. NO is a messenger molecule with diverse functions throughout the body including the maintenance of vascular integrity, homeostasis, synaptic plasticity, long-term potentiation, learning, and memory.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Nitric oxide synthase, brain; Constitutive NOS; NC-NOS; NOS type I; Neuronal NOS; N-NOS; nNOS; Peptidyl-cysteine S-nitrosylase NOS1; bNOS; NOS1

Swiss Prot

P29475

Reactivity

Human

Immunogen

Synthetic phosphopeptide derived from human NOS1 around the phosphorylation site of Serine 1417

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 160 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-NOS1 (S1417) pAb detects endogenous levels of p-NOS1 (S1417) protein only when phosphorylated at Ser1417.

Applications Notes

Western blot (WB) analysis of p-NOS1 (S1417) pAb at 1:500 dilution Lane1:PC3 whole cell lysate (40ug) Lane2:U-87MG whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug)