Catenin-β (phospho-Y670) polyclonal antibody
Product Specifications
Background
β-Catenin is a key downstream effector in the Wnt signaling pathway. It is implicated in two major biological processes in vertebrates: early embryonic development and tumorigenesis. CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β. GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41. Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Catenin beta-1; Beta-catenin; CTNNB1; CTNNB; OK/SW-cl.35; PRO2286
Swiss Prot
P35222
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human CTNNB1 around the phosphorylation site of Tyrosine 670.
Conjugation
Unconjugated
Related Pathways
Hypoxia Signaling, Wntβ catenin signaling, Contribution of ECM and Cytoskeletal Factors to EMT, Contribution of Soluble Factors to EMT, hippo Signaling, Adherens Junction signaling
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 85 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Catenin-β (phospho-Y670) polyclonal antibody detects endogenous levels of Catenin-β protein only when phosphorylated at Tyr670.
Applications Notes
Western blot (WB) analysis Catenin-β (phospho-Y670) polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate (40ug) Lane2:HepG2 whole cell lysate (40ug) Lane3:AML-12 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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