BLK (phospho-Y389) polyclonal antibody

Product Specifications

Background

Blk is a Src family protein tyrosine kinase expressed in all stages of B cell development. Activation of B cells by various ligands is accompanied by activation of Blk. It has been suggested that Blk is involved in the control of B cell differentiation and proliferation. Blk transcripts have also been detected in human thymocytes, but not in mature T cells, implicating that Blk may play an important role in thymopoiesis. Blk function may be redundant, however, as mice that do not express Blk are not impaired with respect to B cell development and immune response.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Tyrosine-protein kinase Blk; B lymphocyte kinase; p55-Blk; BLK

Swiss Prot

P51451

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human BLK around the phosphorylation site of Tyrosine389.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 57 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

BLK (phospho-Y389) polyclonal antibody detects endogenous levels of BLK protein only when phosphorylated at Tyr389 .

Applications Notes

Western blot (WB) analysis of BLK (phospho-Y389) polyclonal antibody at 1:500 dilution Lane1:COS-7whole cell lysate (40ug) Lane2:MCF-7whole cell lysate (40ug) Lane3:CT26 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug)