BLK (phospho-Y389) polyclonal antibody
Product Specifications
Background
Blk is a Src family protein tyrosine kinase expressed in all stages of B cell development. Activation of B cells by various ligands is accompanied by activation of Blk. It has been suggested that Blk is involved in the control of B cell differentiation and proliferation. Blk transcripts have also been detected in human thymocytes, but not in mature T cells, implicating that Blk may play an important role in thymopoiesis. Blk function may be redundant, however, as mice that do not express Blk are not impaired with respect to B cell development and immune response.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Tyrosine-protein kinase Blk; B lymphocyte kinase; p55-Blk; BLK
Swiss Prot
P51451
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human BLK around the phosphorylation site of Tyrosine389.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 57 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
BLK (phospho-Y389) polyclonal antibody detects endogenous levels of BLK protein only when phosphorylated at Tyr389 .
Applications Notes
Western blot (WB) analysis of BLK (phospho-Y389) polyclonal antibody at 1:500 dilution Lane1:COS-7whole cell lysate (40ug) Lane2:MCF-7whole cell lysate (40ug) Lane3:CT26 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
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