ERBB3 (phospho-Y1328) polyclonal antibody

The EGF receptor family comprises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas. Members of this receptor family include EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3) and ErbB-4 (HER4), which form either homodimers or heterodimers upon ligand binding. Full length ErbB-3 is overexpressed in human mammary tumors. The ErbB-3 gene also produces several alternative variants, including a secreted form of which negatively regulates heregulin stimulated ErbB activation. ErbB-3 heterodimerizes with Neu and binds heregulin in order to activate phosphoinositide (PI) 3-kinase. The recruitment and activation of PI 3-kinase occurs via its interaction with phosphorylated YXXM motifs in the carboxy terminus of ErbB-3.

Receptor tyrosine-protein kinase erbB-3 (EC:2.7.10.1) ; Proto-oncogene-like protein c-ErbB-3; Tyrosine kinase-type cell surface receptor HER3; ERBB3; HER3

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of p-ERBB3 (Y1328) polyclonal antibody pAb at 1:500 dilution Lane1:MCF-7 treated with EGF (0.1ng/m) 30 min whole cell lysate Lane2:THP-1 treated with EGF (0.1ng/m) 30 min whole cell lysate Lane3:Raw264.7 treated with EGF (0.1ng/m) 30 min whole cell lysate