EPHA7 (phospho-Y608) polyclonal antibody
Product Specifications
Background
The Eph subfamily represents the largest group of receptor protein tyrosine kinases identified to date. The Eph subfamily receptors of human origin (and their murine/avian homologs) include EphA1 (Eph), EphA2 (Eck), EphA3 (Hek4), EphA4 (Hek8), EphA5 (Hek7), EphA6 (Hek12), EphA7 (Hek11/MDK1), EphA8 (Hek3), EphB1 (Hek6), EphB2 (Hek5), EphB3 (Cek10, Hek2), EphB4 (Htk), EphB5 (Hek9) and EphB6 (Mep) . EphAs are a family of receptor tyrosine kinases that are involved in axonal guidance during development. These receptors and their ligands, the ephrins, act via repulsive mechanisms to guide growing axons towards their appropriate targets and allow for the correct developmental connections to be made. Ligand binding to an Eph receptor results in tyrosine phosphorylation of the kinase domain, and repulsion of axonal growth cones and migrating cells. During neurulation, ephrin-A5 is coexpressed with its cognate receptor EphA7 in cells at the edges of the dorsal neural folds. Three different EphA7 splice variants, a full-length form and two truncated versions lacking kinase domains, are expressed in the neural folds.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Ephrin type-A receptor 7; EPH homology kinase 3; EHK-3; EPH-like kinase 11; EK11; hEK11; EPHA7; EHK3; HEK11
Swiss Prot
Q15375
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to residues in Human EPHA7 around the phosphorylation site of Tyr608.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 112 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-EPHA7 (Y608) polyclonal antibody detects endogenous levels of EPHA7 protein only when phosphorylated at Tyr608.
Applications Notes
Western blot (WB) analysis of p-EPHA7 (Y608) polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate Lane2:sp2/0 whole cell lysate Lane3:PC12 whole cell lysate
Host or Source
Rabbit
Available Sizes
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