TSC2 (phospho-T1462) polyclonal antibody

Product Specifications

Background

Tuberous sclerosis (TSC) is a human genetic disorder characterized by mental retardation and the widespread development of benign and infrequently malignant tumors in a variety of tissues. Two different genetic loci have been linked to TSC; one of these loci, the tuberous sclerosis-2 gene (TSC2), encodes a protein 1784 amino acids in length, called tuberin. Tuberin exhibits a region of limited homology to the catalytic domain of Rap1 GAP. Subcellular fractionation studies have shown tuberin to be predominantly localized in membrane fractions. Tuberin is capable of stimulating the intrinsic GTPase activity of Rap 1A, but not Rap 2, H-Ras, Rac or Rho. TSC2 maps to human chromosome 16 and is associated with several intragenic mutations in affected patients. The mouse homolog of the tuberin gene maps to chromosome 17.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Tuberin; Tuberous sclerosis 2 protein; TSC2; TSC4

Swiss Prot

P49815

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to residues in Human TSC2 around the phosphorylation site of Thr1462.

Conjugation

Unconjugated

Related Pathways

PI3K AKT signaling, Insulin &Glucose Signaling, MTOR signaling

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 180 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

TSC2 (phospho-T1462) polyclonal antibody detects endogenous levels of TSC2 protein only when phosphorylated at Thr1462.

Applications Notes

Western blot (WB) analysis of p-TSC2 (T1462) polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate Insulin (30ng/ml,5min) Lane2:H9C2 cell lysateEGF (20ng/ml,30min) Lane3:Raw264.7 cell lysate EGF (20ng/ml,30min)