Bim (phospho-S69/S65) polyclonal antibody
Product Specifications
Background
Pro-apototic Bcl-2 family members promote cell death by neutralizing their anti-apoptotic relatives, which otherwise maintain cell viability by regulating caspase activity. Bim belongs to the BH3-only subgroup of Bcl-2 related proteins, and exists in three distinct isoforms, BimS (short), BimL (long) and BimEL (extra long) . ERK1/2 phosphorylates BimEL, resulting in rapid degradation of the isoform via the proteasome pathway. At least three sites for ERK1/2 phosphorylation exist on BimEL, whereas ERK1/2 does not effect BimS and BimL, implying a unique role for BimEL in cell survival signaling.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Bcl-2-like protein 11; Bcl2-L-11; Bcl2-interacting mediator of cell death; BCL2L11; BIM
Swiss Prot
O43521
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to residues in Human Bim around the phosphorylation site of Ser69.
Conjugation
Unconjugated
Related Pathways
Regulation of Apoptosis, Inhibition of Apoptosis, Mitochondrial Control of Apoptosis
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 25 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -22°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-Bim (S69/S65) polyclonal antibody detects endogenous levels of human Bim protein only when phosphorylated at Ser69. This antibody also recognizes mouse or rat Bim Protein when phosphorylated at Ser65.
Applications Notes
Western blot (WB) analysis of p-Bim (S69/S65) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:sp2/0 whole cell lysate
Host or Source
Rabbit
Available Sizes
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