EIF2S2 (phospho-S67) polyclonal antibody

Product Specifications

Background

The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF2 exists as a heterotrimeric complex of eIF2α, eIF2ß and eIF2γ. eIF2 functions in the early stages of protein synthesis, by forming a ternary complex with GTP and tRNA. This complex binds to the 40S ribosomal subunit, followed by mRNA binding to 40S to form the 43S preinitiation complex, the release of eIF2 from 40S, and the hydrolysis of GTP. Phosphorylation of eIF2a correlates with inhibition of translation initiation .

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Eukaryotic translation initiation factor 2 subunit 2; Eukaryotic translation initiation factor 2 subunit beta; eIF-2-beta; EIF2S2; EIF2B

Swiss Prot

P20042

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to residues in Human EIF2S2 around the phosphorylation site of ser67.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 38 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-EIF2S2 (S67) polyclonal antibody detects endogenous levels of EIF2S2 protein only when phosphorylated at Ser67.

Applications Notes

Western blot (WB) analysis of p-EIF2S2 (S67) polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate Lane2:sp2/0 whole cell lysate Lane3:PC12 whole cell lysate