P53 (Acetyl-K386) polyclonal antibody

Product Specifications

Background

P53, a DNA-binding, oligomerization domain- and transcription activation domain-containing tumor suppressor, upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation, and cell cycle control mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator, MDM2. MDM2 is an E3 ubiquitin ligase that is upregulated in the presence of active p53, where it poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between latent and active DNA-binding conformations and is differentially activated through posttranslational modifications, including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) of p53, amino acids 110-286, can compromise energetically-favorable association with cis elements and are implicated in several human cancers.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53; TP53; P53

Swiss Prot

P04637

Reactivity

Human, Rat

Immunogen

A synthetic peptide corresponding to residues in Human TP53 around the acetylated site of Lys386.

Conjugation

Unconjugated

Related Pathways

Regulation of Apoptosis, Hypoxia Signaling, Warburg Effect, Signaling Pathways Activating p38 MAP KinaseCell Cycle Control G1 S Checkpoint, Examples of Crosstalk Between Post-translational Modifications, Cell Cycle G2 M DNA Damage Signaling Pathway

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Acetylated

Molecular Weight

~ 53 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Ac-p53 (K386) polyclonal antibody detects endogenous levels of human p53 protein only when acetylated at Lys386. This antibody also recognizes mouse and rat p53 protein when acetylated at the corresponding residues.

Applications Notes

Western blot (WB) analysis of p53 (Acetyl-K386) polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate treated with colchicine (0.2ng/ml,24h) Lane2:Hela cell lysate treated with colchicine (0.2ng/ml,24h) Lane3:H9C2 cell lysate treated with colchicine (0.2ng/ml,24h)