ATM (phospho-S1981) polyclonal antibody
Product Specifications
Background
The phosphatidylinositol kinase (PIK) family members fall into two distinct subgroups. The first subgroup contains proteins such as the PI 3- and PI 4-kinases and the second group comprises the PIK-related kinases. The PIK-related kinases include Atm, DNA-PKCS and FRAP. These proteins have in common a region of homology at their carboxy-termini that is not present in the PI 3- and PI 4-kinases. The Atm gene is mutated in the autosomal recessive disorder ataxia telangiectasia (AT) that is characterized by cerebellar degeneration (ataxia) and the appearance of dilated blood vessels (telangiec-tases) in the conjunctivae of the eyes. AT cells are hypersensitive to ionizing radiation, impaired in mediating the inhibition of DNA synthesis and display delays in p53 induction.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Serine-protein kinase ATM; Ataxia telangiectasia mutated; A-T mutated; ATM
Swiss Prot
Q13315
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to residues in Human ATM around the phosphorylation site of ser1981.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 370 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-ATM (S1981) polyclonal antibody detects endogenous levels of ATM protein only when phosphorylated at ser1981.
Applications Notes
Western blot (WB) analysis of p-ATM (S1981) polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate treated with colchicine (0.2ng/ml,24h) Lane2:Raw264.7 whole cell lysate treated with colchicine (0.2ng/ml,24h) Lane3:H9C2 whole cell lysate treated with colchicine (0.2ng/ml,24h)
Host or Source
Rabbit
Available Sizes
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