PDGFR-β (phospho-Y1009) polyclonal antibody
Product Specifications
Background
PDGF is amitogen formesenchyme- and glia-derived cells. Itconsists of two disulfide-bonded polypeptide chains, A and B, and occurs asthree isoforms, PDGF-AA, PDGF-AB and PDGF-BB. The three isoforms bindwith different affinitiesto two receptor types, A and B, which are structurallyrelated and possess protein-tyrosine kinase domains.Ligand binding induces activation of the receptorkinases byformation of receptor dimers; the A subunit of PDGF binds onlyto A receptorswith high affinity, whereasthe B subunitcan bind to both A and B receptors. Evidence suggeststhat PDGFmayfunction as a neurotrophicfactor. The fact that PDGFtype A receptors are expressed in oligodendrocyte progenitorcellswhereas PDGFtype B receptors are expressed on neuronssuggeststhat the different isoforms of PDGF may regulate growth and differentiation of differentcell typesin the developing central nervoussystemby paracrine and autocrine routes
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Platelet-derived growth factor receptor beta; PDGF-R-beta; PDGFR-beta; EC=2.7.10.1; Beta platelet-derived growth factor receptor Beta-type platelet-derived growth factor receptor CD140 antigen-like family member B; Platelet-derived growth factor receptor 1; PDGFR-1; CD140b; PDGFRB; PDGFR, PDGFR1
Swiss Prot
P09619
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to residues in Human PDGFR-β around the phosphorylation site of Tyrosine 1009.
Conjugation
Unconjugated
Related Pathways
Tumor Angiogenesis
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 190 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-PDGFR-β (Y1009) polyclonal antibody detects endogenous levels of PDGFR-β protein only when phosphorylated at Tyr1009.
Applications Notes
Western blot (WB) analysis of p-PDGFR-β (Y1009) polyclonal antibody at 1:500 dilution Lane1:A549 cell lysate treated with EGF (0.1ng/ml,30mins) Lane2:Raw264.7 cell lysate treated with EGF (0.1ng/ml,30mins) Lane3:PC12 cell lysate treated with EGF (0.1ng/ml,30mins)
Host or Source
Rabbit
CAS Number
9007-83-4
Available Sizes
Curated Selection
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