PDGFR-β (phospho-Y1009) polyclonal antibody

PDGF is amitogen formesenchyme- and glia-derived cells. Itconsists of two disulfide-bonded polypeptide chains, A and B, and occurs asthree isoforms, PDGF-AA, PDGF-AB and PDGF-BB. The three isoforms bindwith different affinitiesto two receptor types, A and B, which are structurallyrelated and possess protein-tyrosine kinase domains.Ligand binding induces activation of the receptorkinases byformation of receptor dimers; the A subunit of PDGF binds onlyto A receptorswith high affinity, whereasthe B subunitcan bind to both A and B receptors. Evidence suggeststhat PDGFmayfunction as a neurotrophicfactor. The fact that PDGFtype A receptors are expressed in oligodendrocyte progenitorcellswhereas PDGFtype B receptors are expressed on neuronssuggeststhat the different isoforms of PDGF may regulate growth and differentiation of differentcell typesin the developing central nervoussystemby paracrine and autocrine routes

Platelet-derived growth factor receptor beta; PDGF-R-beta; PDGFR-beta; EC=2.7.10.1; Beta platelet-derived growth factor receptor Beta-type platelet-derived growth factor receptor CD140 antigen-like family member B; Platelet-derived growth factor receptor 1; PDGFR-1; CD140b; PDGFRB; PDGFR, PDGFR1

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of p-PDGFR-β (Y1009) polyclonal antibody at 1:500 dilution Lane1:A549 cell lysate treated with EGF (0.1ng/ml,30mins) Lane2:Raw264.7 cell lysate treated with EGF (0.1ng/ml,30mins) Lane3:PC12 cell lysate treated with EGF (0.1ng/ml,30mins)