PDGFR-β (phospho-Y1009) polyclonal antibody

Product Specifications

Background

PDGF is amitogen formesenchyme- and glia-derived cells. Itconsists of two disulfide-bonded polypeptide chains, A and B, and occurs asthree isoforms, PDGF-AA, PDGF-AB and PDGF-BB. The three isoforms bindwith different affinitiesto two receptor types, A and B, which are structurallyrelated and possess protein-tyrosine kinase domains.Ligand binding induces activation of the receptorkinases byformation of receptor dimers; the A subunit of PDGF binds onlyto A receptorswith high affinity, whereasthe B subunitcan bind to both A and B receptors. Evidence suggeststhat PDGFmayfunction as a neurotrophicfactor. The fact that PDGFtype A receptors are expressed in oligodendrocyte progenitorcellswhereas PDGFtype B receptors are expressed on neuronssuggeststhat the different isoforms of PDGF may regulate growth and differentiation of differentcell typesin the developing central nervoussystemby paracrine and autocrine routes

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Platelet-derived growth factor receptor beta; PDGF-R-beta; PDGFR-beta; EC=2.7.10.1; Beta platelet-derived growth factor receptor Beta-type platelet-derived growth factor receptor CD140 antigen-like family member B; Platelet-derived growth factor receptor 1; PDGFR-1; CD140b; PDGFRB; PDGFR, PDGFR1

Swiss Prot

P09619

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to residues in Human PDGFR-β around the phosphorylation site of Tyrosine 1009.

Conjugation

Unconjugated

Related Pathways

Tumor Angiogenesis

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 190 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-PDGFR-β (Y1009) polyclonal antibody detects endogenous levels of PDGFR-β protein only when phosphorylated at Tyr1009.

Applications Notes

Western blot (WB) analysis of p-PDGFR-β (Y1009) polyclonal antibody at 1:500 dilution Lane1:A549 cell lysate treated with EGF (0.1ng/ml,30mins) Lane2:Raw264.7 cell lysate treated with EGF (0.1ng/ml,30mins) Lane3:PC12 cell lysate treated with EGF (0.1ng/ml,30mins)