Myc-Tag polyclonal antibody
Product Specifications
Background
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da) . It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Myc, c Myc Epitope Tag, c Myc Tag, Myc Epitope Tag, Myc proto-oncogene protein, Transcription factor p64
Reactivity
Transfected
Immunogen
Synthetic peptide Myc-Tag.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:2000~1:5000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
The antibody detects over-expressed or recombinant proteins containing the Myc epitope tag.
Applications Notes
Western blot (WB) analysis of Myc Tag polyclonal antibody at 1:2000 dilution Lane1:c-Myc-GST tagged protein Lane2:HEK293T whole cell lysate
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
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