PAPOLA polyclonal antibody

Polyadenylation of the 3' ends of eukaryotic mRNAs is a key event that takes place in the nucleus during maturation of mRNA. The reaction occurs in two distinct steps: endoribonucleolytic cleavage of the pre-RNA at the poly (A) site, followed by synthesis of the poly (A) tail at the 3' end of the up-stream cleavage product. The poly (A) polymerase (PAP) is required for the adenosine addition reaction. Western blot analysis reveals three PAPs, namely PAP-α, PAP-β and PAP-γ, demonstrating different molecular masses in HeLa cell extracts. The amino-terminal region of PAP is required for nonspecific polymerase activity, while both the amino and carboxy termini are required for specific polymerase activity. Additionally, PAP contains a functional ribonucleoprotein-type RNA binding domain (RBD) that is responsible for primer binding.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of PAPOLA pAb at 1:1000 dilution Lane1:HCC827 whole cell lysate (40ug) Lane2:The Embryo tissue lysate of Mouse (40ug) Lane3:PMVEC whole cell lysate (40ug) Lane4:EC9706 whole cell lysate (40ug)