PAPOLA polyclonal antibody
Product Specifications
Background
Polyadenylation of the 3' ends of eukaryotic mRNAs is a key event that takes place in the nucleus during maturation of mRNA. The reaction occurs in two distinct steps: endoribonucleolytic cleavage of the pre-RNA at the poly (A) site, followed by synthesis of the poly (A) tail at the 3' end of the up-stream cleavage product. The poly (A) polymerase (PAP) is required for the adenosine addition reaction. Western blot analysis reveals three PAPs, namely PAP-α, PAP-β and PAP-γ, demonstrating different molecular masses in HeLa cell extracts. The amino-terminal region of PAP is required for nonspecific polymerase activity, while both the amino and carboxy termini are required for specific polymerase activity. Additionally, PAP contains a functional ribonucleoprotein-type RNA binding domain (RBD) that is responsible for primer binding.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Poly (A) polymerase alpha; PAP-alpha; Polynucleotide adenylyltransferase alpha; PAPOLA; PAP
Swiss Prot
P51003
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to Human PAPOLA.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 100 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
PAPOLA pAb detects endogenous levels of PAPOLA protein.
Applications Notes
Western blot (WB) analysis of PAPOLA pAb at 1:1000 dilution Lane1:HCC827 whole cell lysate (40ug) Lane2:The Embryo tissue lysate of Mouse (40ug) Lane3:PMVEC whole cell lysate (40ug) Lane4:EC9706 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
Curated Selection
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