PAPOLA polyclonal antibody

Product Specifications

Background

Polyadenylation of the 3' ends of eukaryotic mRNAs is a key event that takes place in the nucleus during maturation of mRNA. The reaction occurs in two distinct steps: endoribonucleolytic cleavage of the pre-RNA at the poly (A) site, followed by synthesis of the poly (A) tail at the 3' end of the up-stream cleavage product. The poly (A) polymerase (PAP) is required for the adenosine addition reaction. Western blot analysis reveals three PAPs, namely PAP-α, PAP-β and PAP-γ, demonstrating different molecular masses in HeLa cell extracts. The amino-terminal region of PAP is required for nonspecific polymerase activity, while both the amino and carboxy termini are required for specific polymerase activity. Additionally, PAP contains a functional ribonucleoprotein-type RNA binding domain (RBD) that is responsible for primer binding.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Poly (A) polymerase alpha; PAP-alpha; Polynucleotide adenylyltransferase alpha; PAPOLA; PAP

Swiss Prot

P51003

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to Human PAPOLA.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 100 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

PAPOLA pAb detects endogenous levels of PAPOLA protein.

Applications Notes

Western blot (WB) analysis of PAPOLA pAb at 1:1000 dilution Lane1:HCC827 whole cell lysate (40ug) Lane2:The Embryo tissue lysate of Mouse (40ug) Lane3:PMVEC whole cell lysate (40ug) Lane4:EC9706 whole cell lysate (40ug)