NANOG polyclonal antibody
Product Specifications
Background
Nanog is a homeodomain-containing transcription factor that is essential for the maintenance of pluripotency and self renewal in embryonic stem cells. Nanog expression is controlled by a network of factors including Sox2 and the key pluripotency regulator Oct-4. Recent advances in somatic cell reprogramming have utilized viral expression of combinations of transcription factors including nanog, Oct-4, Sox2, KLF4, c-Myc, and LIN28.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Homeobox protein NANOG; Homeobox transcription factor Nanog; hNanog; NANOG; Putative homeobox protein NANOG2; NANOGP1; NANOG2
Swiss Prot
Q9H9S0/Q8N7R0
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding Human NANOG.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:1000~1:5000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 34 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
NANOG pAb detects endogenous levels of NANOG protein.
Applications Notes
Western blot (WB) analysis of NANOG pAb at 1:2000 dilution Lane1:A2780 whole cell lysate (10ug) Lane2:Hela whole cell lysate (10ug) Lane3:3T3-L1 whole cell lysate (10ug) Lane4:The Testis tissue lysate of Rat (10ug)
Host or Source
Rabbit
Available Sizes
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