ACR polyclonal antibody

Product Specifications

Background

Acrosin, a member of the peptidase S1 family, is a major protease present in the acrosome of mature mammalian spermatozoa. Acrosin is a typical serine proteinase with trypsin-like cleavage specificity. The zymogen form, proacrosin, is the precursor of Acrosin synthesized only in the postmeiotic stages of spermatogenesis. The active enzyme functions in the lysis of the zona pellucida, allowing the penetration of sperm through the innermost glycoprotein layers of the ovum.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Acrosin; Acrosin light chain; Acrosin heavy chain; ACR; ACRS

Swiss Prot

P10323

Reactivity

Human

Immunogen

Synthetic peptide, corresponding to amino acids 1-50 of Human ACR.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 46 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

ACR polyclonal antibody detects endogenous levels of ACR protein.

Applications Notes

Western blot (WB) analysis of ACR polyclonal antibody at 1:500 dilution Lane1:HepG2 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:MCF-7 whole cell lysate (40ug) Lane4:L02 whole cell lysate (40ug)